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Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample datadheet available.
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It should be noted that for the best datasheeet results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal.
Additionally, it is recommended that you verify the removal of the first vatasheet complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary vatasheet. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Do not aliquot the antibody.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn of human insulin receptor protein.
Insulin receptor InsR is a heterodimeric membrane receptor tyrosine kinase. Binding of insulin to InsR results in receptor autophosphorylation, datasheef subsequent tyrosine kinase activation 2.
These events lead to increased glucose uptake and metabolism, and can promote cell growth. Loss of function mutation or desensitization of the InsR are two major contributors to insulin resistance and Type datashet diabetes 5. Changing to another country might result in loss of shopping cart. Would you like to visit your country specific website?
Insulin Receptor α (D3U7I) Rabbit mAb #74118
More about how we get our images. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.
Dilute to 1X with dH 2 O. Primary Antibody Dilution Buffer: Biotinylated Protein Ladder Detection Pack: Blotting Membrane and Paper: Protein Blotting A general protocol for sample preparation.
Treat cells by adding fresh media containing regulator for desired time. Aspirate media 741188 cultures; wash cells with 1X PBS; datasbeet.
Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Microcentrifuge for 5 min. Electrotransfer to nitrocellulose membrane Incubate membrane in 25 ml of blocking buffer for 1 hr at datasgeet temperature.
Wash three times for 5 min each with 15 ml of TBST. Proceed with detection Section D. Detection of Proteins Directions for Use: Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film.
Application Dilutions Western Blotting 1: To Purchase S View sizes. Find answers on our FAQs page.