DBM – 21S PDF

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In some strains of Saccharomyces cerevisiae the mitochondrial dbn coding for 21S rRNA is interrupted by an intron of bp. This intron contains a reading frame for amino acids: In order dgm check whether expression of this URF is required for proper splicing of precursors to 21S rRNA, the precision of RNA splicing was analysed in a petite mutant, where no mitochondrial protein synthesis is possible anymore.

We have devised a new assay to monitor the precision of the splicing event. The method is of general application, provided that the sequence of the splice boundaries is known. Resistance to digestion will only be observed if the correct splice-junction is made. In RNA blotting experiments, using an intron specific hybridisation probe, the same intermediates in splicing are found both in wild type and petite mutant. Finally the synthetic oligonucleotide hybridises to petite 21S rRNA and its thermal dissociation behaviour is indistinguishable from a hybrid formed with wildtype 21S rRNA.

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Close mobile search navigation Article navigation. Abstract In some dbmm of Saccharomyces cerevisiae the mitochondrial gene coding for 21S rRNA is interrupted by an intron of bp.

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Email alerts New issue alert. Receive exclusive offers and updates from Oxford Academic. More on this topic Comparison of intron-containing and intron-lacking human genes elucidates putative exonic splicing enhancers. Splicing of many human genes involves sites embedded 21w introns. Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro.

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